Sporadic multiple meningiomas harbor distinct driver mutations

© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/publi cdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. In a small fraction of patients, intracranial meningiomas arise as multiple and spatially distinct masses therefore presenting a unique management challenge [9, 12, 19]. A recently-published, (Surveillance, Epidemiology, and End Results) SEER-based study has reported that patients with multiple meningiomas (MM) have substantially reduced overall survival when compared to patients with single meningiomas [14]. Patients may develop multiple meningiomas in sporadic or hereditary forms. Familial syndromes that are commonly associated with MM are neurofibromatosis type 2 (NF2) and familial meningiomatosis in patients with germline NF2 and SMARCB1 mutations, respectively [2, 15]. While the mutational landscape of single meningiomas has been extensively studied [3–5, 10, 20], understanding of the molecular pathogenesis of sporadic MM remains incomplete. Older studies and case reports have reported molecular testing in patients with sporadic MM that have principally been focused on tumors with NF2 mutations [8, 16–18]. However, to our knowledge, no molecular profiling in a case series of spatially separated MM, composed of different histological subtypes, has been performed. The objective of this study is to elucidate the genetic features of sporadic MM, defined as the presence of ≥ 2 spatially separated synchronous or metachronous lesions. This series includes 17 resected sporadic meningiomas from eight patients (seven females and one male) that were identified by a record search for patients with MM. All patients presented with synchronous, spatially separated meningiomas without evidence of tumor bridging, as reviewed on MR-imaging. The patients had no significant prior radiation exposure and the tumors did not arise in patients who met the clinical criteria for the diagnosis of familial schwannomatosis or neurofibromatosis type 2 [6]. In addition, upon reviewing cranial and spinal MR images, no patient had other intraor extracranial tumors associated with hereditary meningioma syndromes such as schwannomas or ependymomas. Fresh frozen tumor tissue was available from all 17 meningiomas and was retrieved from the archives of the Institute for Pathology at the University Hospital Dresden upon approval of the local ethics committee. Two board-certified pathologists confirmed the pathologic diagnosis of each case. All tumors were classified according to the 2016 WHO classification of tumors of the central nervous system [11]. The tumor DNA was purified using AllPrep® DNA Universal Kit for fresh frozen tissue (Qiagen, Germantown MD) following the manufacturer’s instructions. The regions of interest were amplified using a custom designed amplikon panel according to the protocol “QIAseq Targeted DNA V3 Panel, May 2017” (QIAGEN, Hilden, Germany). The panel was custom-designed by our group and manufactured by QIAGEN. The panel covers either mutation hotspots or—where loss of function is a known mechanism of action—whole genes. The following meningioma-relevant genes are included: AKT1, ATRX, CDKN2A, KLF4, NF1, NF2, PIK3CA, PIK3R1, POLR2A, PTEN, SMARCB1, SMO, STAG2, SUFU, TP53, TRAF7, and TERT promotor. Open Access

In a small fraction of patients, intracranial meningiomas arise as multiple and spatially distinct masses therefore presenting a unique management challenge [9,12,19]. A recently-published, (Surveillance, Epidemiology, and End Results) SEER-based study has reported that patients with multiple meningiomas (MM) have substantially reduced overall survival when compared to patients with single meningiomas [14]. Patients may develop multiple meningiomas in sporadic or hereditary forms. Familial syndromes that are commonly associated with MM are neurofibromatosis type 2 (NF2) and familial meningiomatosis in patients with germline NF2 and SMARCB1 mutations, respectively [2,15]. While the mutational landscape of single meningiomas has been extensively studied [3-5, 10, 20], understanding of the molecular pathogenesis of sporadic MM remains incomplete. Older studies and case reports have reported molecular testing in patients with sporadic MM that have principally been focused on tumors with NF2 mutations [8,[16][17][18]. However, to our knowledge, no molecular profiling in a case series of spatially separated MM, composed of different histological subtypes, has been performed. The objective of this study is to elucidate the genetic features of sporadic MM, defined as the presence of ≥ 2 spatially separated synchronous or metachronous lesions.
This series includes 17 resected sporadic meningiomas from eight patients (seven females and one male) that were identified by a record search for patients with MM. All patients presented with synchronous, spatially separated meningiomas without evidence of tumor bridging, as reviewed on MR-imaging. The patients had no significant prior radiation exposure and the tumors did not arise in patients who met the clinical criteria for the diagnosis of familial schwannomatosis or neurofibromatosis type 2 [6]. In addition, upon reviewing cranial and spinal MR images, no patient had other intra-or extracranial tumors associated with hereditary meningioma syndromes such as schwannomas or ependymomas.
Fresh frozen tumor tissue was available from all 17 meningiomas and was retrieved from the archives of the Institute for Pathology at the University Hospital Dresden upon approval of the local ethics committee. Two board-certified pathologists confirmed the pathologic diagnosis of each case. All tumors were classified according to the 2016 WHO classification of tumors of the central nervous system [11]. The tumor DNA was purified using AllPrep ® DNA Universal Kit for fresh frozen tissue (Qiagen, Germantown MD) following the manufacturer's instructions. The regions of interest were amplified using a custom designed amplikon panel according to the protocol "QIAseq Targeted DNA V3 Panel, May 2017" (QIAGEN, Hilden, Germany). The panel was custom-designed by our group and manufactured by QIAGEN. The panel covers either mutation hotspots or-where loss of function is a known mechanism of action-whole genes. The following meningioma-relevant genes are included: AKT1, ATRX, CDKN2A, KLF4, NF1, NF2, PIK3CA, PIK3R1, POLR2A, PTEN, SMARCB1, SMO, STAG2, SUFU, TP53, TRAF7, and TERT promotor.

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*Correspondence: tareq.juratli@uniklinikum-dresden.de 1 Department of Neurosurgery, University Hospital Carl Gustav Carus, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany Full list of author information is available at the end of the article During library preparation unique molecular barcodes and sample specific indices were incorporated according to the protocol. Indexed libraries were then quantified using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, MA, USA) and paired end sequenced (2x200 bp) on Illumina MiSeq platform. HG19 was used as reference genome for bioinformatic analyses. The bioinformatics evaluation was performed using the Biomedical Workbench from CLC (12.0.3) using a customized analysis algorithm with following filters: coverage >/=100, allele frequency >/=5%. Notably, we performed internal NGS controls for identity check and cross contamination checks to assure the assignment of the correct samples.
The average age at presentation was 60 years (range 43-75 years) which is comparable with the age of patients with single sporadic meningiomas [9]. Six patients (75%) underwent two surgeries within 2 years for tumor resection, whereas in two patients the meningiomas were removed at the same time (patients 1 and 7). Fourteen meningiomas were WHO grade 1 (82.3%) and the remaining three tumors were WHO grade 2. This is consistent with previous reports of the predominance of WHO grade 1 among MM [13,19].
Most importantly, the same mutation was not identified in separate tumors from the same patient, suggesting genomically distinct molecular drivers and an independent origin of these multiple lesions. All but two cases harbored TRAF7, AKT1, SMO or PIK3CA mutations (Fig. 1). The most frequent driver mutations in our series were TRAF7 (n = 5), PIK3CA H1047R and E545G (n = 3), AKT1 E17K (n = 3), NF2 (n = 2), SMO L412F (one case) and NF1 (one case). We did not detect a known driver mutation in only one meningioma (MM #3, Site B; Table 1). Interestingly, with the exception of one patient (MM #5), all tumors from the same patient were different histopathological subtypes ( Table 1).
The low frequency of NF2 mutations in our MM series stands in contrast to previous studies that included hereditary cases arising in the setting of NF2 [2,15,16,18]. Those studies identified a high prevalence of NF2 mutations (up to 83%) and supported a monoclonal origin for MM [8,18]. Our findings in a cohort of 17 MM arising in patients without NF2 support a model in which sporadic MM can arise independently from one another, while a subset of MM may result from somatic NF2 mosaicism [7].
Each of the meningiomas in our study exhibited features that are commonly seen in solitary meningiomas, demonstrating strong associations between the genetic alteration, the histologic subtype and the anatomic location [1,5,21]. The high frequency of known and targetable drivers of meningioma in our cohort suggests that a large fraction of MM may be candidates for study in clinical trials evaluating targeted therapies, such as the ongoing multicenter phase II study (ClinicalTrials.gov NCT02523014) that investigates the efficacy of afuresertib in AKT1-mutant, vismodegib in SMO-mutant and the focal adhesion kinase (FAK) inhibitor GSK2256098 in NF2-mutant meningiomas. Given the inter-tumor and intra-patient heterogeneity that we observe in the setting

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