Fig. 7

Accelerated senescence alters autophagy in vivo. a Western blot analysis showing p62 and LC3 relative protein levels in hippocampal protein extracts from 5-month-old WT, G3Terc^−/−, 5xFAD and G3Terc^−/− 5xFAD mice. Actin was used as loading control, and the levels in the control group were set as 100%. *P < 0.05, **P < 0.01 (One-way ANOVA with Tukey’s post-hoc analysis, n = 7 mice/group). All data are presented as mean ± SEM. b Western blot analysis showing Atg9A and Beclin-1 relative protein levels in hippocampal protein extracts from 5-month-old WT, G3Terc^−/−, 5xFAD and G3Terc^−/− 5xFAD mice. Protein levels were normalized by the amount of Actin, and the levels in the control group were set as 100%. Non-significant (One-way ANOVA with Tukey’s post-hoc analysis, n = 7 mice/group). c Colocalization of Aβ (MOAB2, red) with two different autophagy markers (p62 or LC3, green) in the subiculum of 2-months-old 5xFAD mice. Representative photomicrographs are shown. Scale bar: 10 μm