Fig. 6
Accelerated senescence in 5xFAD mice decreases neuronal density in brain regions presenting intraneuronal Aβ accumulation. a NeuN immunostainings in the subiculum of 2-month-old WT, G3Terc−/−, 5xFAD and G3Terc−/− 5xFAD mice. Representative photomicrographs are shown. **P < 0.01 (One-way ANOVA with Tukey’s post-hoc analysis, n = 3–5). NeuN immunostainings in the subiculum (b) and cortex (c) of 5-month-old WT, G3Terc−/−, 5xFAD and G3Terc−/− 5xFAD mice. Representative photomicrographs are shown. Layer V of the cortex is indicated by dashed lines. Scale bar: 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (One-way ANOVA with Tukey’s post-hoc analysis, n = 4 mice/group). NeuN immunostainings in the CA1 (d) CA3 (e) and DG (f) region of 5-month-old WT, G3Terc−/−, 5xFAD and G3Terc−/− 5xFAD mice. Representative photomicrographs are shown. Scale bar: 100 μm. Non-significant (One-way ANOVA with Tukey’s post-hoc analysis, n = 4 mice/group). The number of NeuN-positive cells per mm2 was calculated as a measure of neuronal density. All data are presented as mean ± SEM