Fig. 1
From: Retina-to-brain spreading of α-synuclein after intravitreal injection of preformed fibrils
Uptake of α-synuclein fibrils following PFFs intravitreal injection. A TEM images of mouse PFFs. Scale bar: 500 nm. B Thioflavin-T intensity of fibrils at day 7 (n = 2 measurements). C CD spectra of α-synuclein monomers and PFFs. D Immunohistochemical staining of total α-synuclein in the retina of PFF-injected mice 1, 3, and 7 days after injection. E–G Quantification of immunoreactivity to α-synuclein in the IPL at 1 day (E), 3-days (F), and 7-days (G) after injection. Scale bar: 50 µm. Data in E, F, G, I and J are expressed as means ± s.e.m. (PBS, n = 5 mice; PFF, n = 5 mice; *p < 0.05, **p < 0.01, ***p < 0.001; Student’s t-test