Table 1 Statistical analysis methods and results for validation data

SPARC + RBFOX1- OLIG2 + (IF)

Linear Model

Cube root (percentage of SPARC + RBFOX1-OLIG2 +) ~ tissue region + age*sex

Predictor

Est

SE

t-value

p-value

NA

BA4: 6

CB: 4

CSC: 6

(Intercept)

1.2

0.3

4.06

 < 0.001

Tissue: CB

− 0.17

0.36

− 0.47

0.64

Tissue: CSC

0.74

0.32

2.34

0.023

Age Group: Young

− 0.43

0.46

− 0.931

0.356

Sex: Male

− 0.02

0.39

− 0.07

0.94

Young: Male

0.35

0.61

0.578

0.565

HCN2 + SPARC + OLIG2 + (IF)

Wilcoxon Rank Sum test

Proportion of HCN2 + SPARC + oligodendrocytes ~ tissue

NA

W = 18.5

p-value = 0.025

BA4: 3

CSC: 2

PAX3 + PDGFRA + (BaseScope duplex)

Poisson Model

Count of double positive cells ~ total count of PDGFRA positive + tissue

(Intercept)

− 1.31

0.34

− 3.82

 < 0.001

NA

5 BA4

5 CB

7 CSC

Total count PDGFRA

0.05

0.02

1.97

0.048

Tissue: CB

1.50

0.31

4.87

 < 0.001

Tissue: CSC

1.65

0.30

5.56

 < 0.001

NELL1 + PDGFRA + (BaseScope duplex)

Linear Model

NELL1 + PDGFRA +  ~ tissue region

(Intercept)

0.67

0.16

4.3

 < 0.001

NA

3 BA4

3 CSC

Tissue: CSC

− 0.67

0.22

− 2.97

0.007

EBF1 + PDGFRA + (RNAscope)

linear model

Proportion EBF1 + PDGFRA +  ~ age group + tissue + mean cellular autofluorescence

(Intercept)

0.43

0.2

4.09

 < 0.001

NA

CB old: 4

CB young: 4

CSC old: 5

CSC young: 5

Age Group

0.18

0.05

3.64

 < 0.001

Tissue (CSC)

− 0.32

0.05

− 7.05

 < 0.0001

Cellular auto-fluorescence

44.00

26.27

1.68

0.099