Fig. 6

Vascular constrictions in the hippocampal region affected by local inflammation. a, Constricted microvasculature with elongated red blood cells in an H&E-stained section. Note the lack of the dark Brite Vu contrast agent used to perfuse this animal within these vessels. b, Higher magnification of microvessel indicated in the box from a. Arrows indicate the highly elongated boxcar-like appearance of red blood cells passing through this constricted microvessel. Panels c and d show abnormal hippocampal arterioles with strictures (arrows) crossing the pyramidal cell layer of CA1. Note the severe microgliosis in this region, as cells with small nuclei are predominant in this region. Dark intravascular substance corresponds to the Brite Vu contrast agent. Insert in d shows higher magnification of the corresponding arteriolar stricture. Scale, 50 µm in a; 20 µm in b; 40 µm in c and d; 10 µm for inset in d