Fig. 11

Hippocampal inflammation is associated with neuronal degeneration and degradation of vascular ECM. Shown are images of a hippocampal region in which a local gliosis that extends through the strata oriens, pyramidale, radiatum and lacunosum moleculare overlaps the dendritic degeneration in stratum radiatum. β-III tubulin (Tuj) (neuronal, green), GFAP (astrocytes, red), collagen type IV (vascular, white), and DAPI (blue) staining. a, merged image; b, reactive astrogliosis (red) overlapping a halo of perivascular collagen type IV immunostaining (white) in the lacunosum moleculare and in large vessels in the oriens (arrows); c, dendritic atrophy associated with inflammation (arrow); d, reactive astrogliosis (red); e, dendritic degeneration (arrow); and f, DAPI staining (blue). Note the small nuclei corresponding to microglia in the region showing dendritic degeneration and reactive astrogliosis. Insert in f shows the contralateral hippocampal region without inflammation (β-III tubulin, green; GFAP red; collagen type IV, white, and DAPI, blue). Scale, 200 µm