Fig. 9

Pediatric low grade glioma bulk transcriptomic data deconvolution in light of snRNA/CITEseq results. A CPTAC pediatric low grade glioma and ganglioglioma bulk RNA-seq deconvolution example using dtangle method with snRNA-seq data cell type reference. B Bergthold et al. low grade glioma bulk RNA-seq deconvolution analogous to A. C Violin plots with CPTAC tumor OPC-like, mixed neuron, and neuroectoderm-like cell composition, by diagnosis. Medians marked with red dots and labeled. Statistical comparisons by Mann Whitney with p values shown. D Analogous to C for Bergthold et al. tumors except for statistical testing, Kruskal − Wallis was used initially, followed by Dunn test with Bonferroni adjustment as needed. OPC = oligodendrocyte precursor cell, DA = diffuse astrocytoma, GG = ganglioglioma, ODG = oligodendroglioma, DNT = dysplastic neuroepithelial tumor, NOS or LGG = low-grade glioma, not otherwise specified. PA = pilocytic astrocytoma