The localization of molecularly distinct microglia populations to Alzheimer's disease pathologies using QUIVER

Table 2 Blocking step optimization

Trial	A&B (drops/ml)	Device	Time (min)	FAB (ug/ml)	Device	Time (hr)	Area fraction (%)	Qualitative evaluation
1	0	–	–	20	S	1	34.305	++++
2	4	S	15	20	S	1	0.066	+
3	6	L	15	20	S	1	0.009	+
4	6	S	60	20	S	1	0.001	–
5	6	L	15	20	S	1	0.005	+
6	6	L	60	20	S	1	0.003	–
7	10	S	15	20	S	1	0.245	+++
8	6	S	60	0	–	–	46.380	++++
9	6	S	60	20	S	1	0.004	+
10	6	S	60	40	S	1	0.007	+
11	6	S	60	40	S	18	0.003	–
12	6	S	60	40	L	1	0.006	+
13	6	S	60	40	L	18	0.009	–
14	6	S	60	60	S	1	0.098	+
15	6	S	60	100	S	1	0.133	++

During the incubations slides were in either Sequenza (S) or lockmailer (L) device. The HALO area fraction module was used to determine the % of stained tissue. An observe blind to the experimental conditions also rated the slides as robust residual staining (++++), some cells present throughout the tissue (+++), a few cells present in select regions (++), faint profiles for staining still observable (+), or no observable staining (–)

ISSN: 2051-5960