Fig. 8

Digital proximity analysis of microglia/macrophage phenotypes to PHF-1⁺ tangles and Aβ⁺ plaques. (A) The Halo software generated markup shows the few cellular profiles within 60 μm of the PHF-1⁺ cell. (B) The Halo proximity analysis defined the relative percentage of the five microglia/macrophage phenotypes at each distance interval from the PHF-1⁺ cell. (C) The average number of cells at the distance intervals away from the PHF-1⁺ cell. (D) Near an Aβ⁺, plaque there is a high density of cells. (E) Nearest the plaque the IBA1 + Ferritin + CD68+ cell, and the IBA1+ phenotypes account for the majority of the plaque-associated cells. (F) The number of microglia/macrophage at the distance interval shows the polarization of marker expression that occurs around 50 μm from the plaque. The results are for 942 and 890 IBA1+ cells for figures A-C and D-F, respectively