Fig. 3

Effects of mIHC protocol on antigenicity following repeated rounds of staining. (A) Serial sections of a Tissue microarray containing human brain samples from individuals with ADRC-NC were stained with rabbit-anti-P2Y12 or guinea pig-anti-IBA1 for the first round (1°) of staining. Digitalizing the side was followed by the refined MICSSS protocol and a second (2°) round of staining. Arrows indicated IBA1⁺P2Y12^– cells. The number of cells in each case on the TMA was quantified using the object colocalization algorithm (HALO 3.4). By a paired t-test, no statistical difference was seen for between 1° or 2° rounds of staining the number of IBA1⁺ cells (B) or P2Y12⁺ cells