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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Loss of MBNL1-mediated retrograde BDNF signaling in the myotonic dystrophy brain

Fig. 3

Loss of MBNL1 impairs DYNLL2 motility in neurons. (a) Time-lapse imaging of the MBNL1 movement in neurons expressing mCherry-tagged MBNL1. A respective kymograph is shown at the bottom. Quantification of the indicated types of movement from a total of 121 neurons is shown at right. (b) Movement of MBNL1ΔEx5 and DYNLL2 in neuronal axons. Anterograde (marked “1”), retrograde (marked “2”) and stationary (marked “3”) movements have been labeled. (c) Time-lapse analysis showing the movement of overexpressed DYNLL2 in MBNL1-depleted neurons. Neurons were transfected with DYNLL2-mCherry, Mbnl1 shRNA (shMbnl1) or control shRNA (pLKO) as indicated. (d) Number of DYNLL2-mCherry movement events categorized into 4 different movement types was listed. Quantification of different DYNLL2-mCherry movements in the control and MBNL1-depleted neurons. Stationary DYNLL2-mCherry is indicated by an arrowhead. Scale bars: a-c, 40 μm. Data are mean ± SEM. *P < 0.05 by Student’s t-test

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