Table 2 Antibodies used for manually performed stainings
From: Cerebellar presence of immune cells in patients with neuro-coeliac disease
Antigen | Antigen Retrival | Species | Dilution | Manufacturer | Article number | Secondary antibody | Manufacturer | Article number | Dilution |
|---|---|---|---|---|---|---|---|---|---|
GFAP | Tris/EDTA Ph9.0 | Rabbit polyclonal | 1:4000 | DAKO | Z0334 | GAR-biot | Jackson | 111–065-144 | 1:400 |
Calbindin | Tris/EDTA Ph9.0 | mouse monoclonal | 1:400 | Swant | 300 | GAM-biot | Jackson | 115–065-146 | 1:400 |
TMEM119 | Tris/EDTA Ph9.0 | Rabbit polyclonal | 1:500 | Atlas | HPA051870 | GAR-biot | Jackson | 115–065-146 | 1:400 |
CD3* | Tris/EDTA Ph9.0 | Rabbit polyclonal | 1:50 | DAKO | A0452 | Alexa 546, Isotype GAR | Life Technologies, Thermo Fisher scientific | A11010 | 1:200 |
CD8* | Tris/EDTA Ph9.0 | Mouse monoclonal | 1:75 | Novocastra, Leica Biosystems | ncl-l-cd8-4b11 | Alexa 647, Isotype GAM IgG2b | Life Technologies, Thermo Fisher scientific | A21242 | 1:200 |
Granzyme B* | Tris/EDTA Ph9.0 | Mouse IgG2a | 1:100 | Invitrogen, Thermo Fisher scientific | MA1-35,461 | A488 Isotype GAM IgG2a | Life Technologies, Thermo Fisher scientific | A21131 | 1:200 |