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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Different MAPT haplotypes influence expression of total MAPT in postmortem brain tissue

Fig. 5

Influence of MAPT haplotype and disease status on insoluble tau protein levels. Bar graphs showing the effect of MAPT haplotypes (H1/H1, H2/H2) and disease status (PD, control) on insoluble protein levels of total tau (A), 0N3R tau (B) and 1N4R tau (C) in human brain samples of the cortex of fusiform gyrus and the cortex of cerebellum. For analysis of the MAPT haplotype effects, PD cases and controls were combined, and vice versa for analysis of the disease status. Data are protein quantity (mean ± SEM) relative to Total Protein Stain and normalized to an inter-run calibration consisting of pooled protein samples. Two-way ANOVA (haplotype × disease status), followed by Tukey’s post-hoc test: *P < 0.05, *d, significant effect of disease. D Representative Western blot for soluble total tau with Total Protein Stain as reference. Each group is represented with 1 donor in each blot. The total independent sample size is: H2/H2 Control n = 5, H2/H2 PD n = 5, H1/H1 Control n = 7, H1/H1 PD n = 9). The six tau isoforms were identified by comparison to a recombinant tau ladder. Full length Western blots are shown in Additional file 2: Figure S5

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