Fig. 5

Brain Aβ deposition in APP/PS1 mice. a, b Representative images showing Aβ deposits in the a hippocampus and b cerebral frontal cortex in APP/Ps1 mice are increased with aging (scale bar 10 µm). c, d Quantification of Aβ burden in c hippocampus and d cerebral cortex from the APP/PS1 mice groups is shown (n = 2–6 mice per group). e Representative images showing the lack of Aβ deposits in the hypothalamic area (scale bar 10 µm). f–h Representative confocal images showing Aβ deposits (green) and neurons immunoslabelled with anti-NeuN (red) in the f hippocampus, g cerebral frontal cortex, and h hypothalamus in APP/PS1 mice (scale bar 50 µm) Inserts with higher magnification showing Aβ accumulated in NeuN⁺ hypothalamic cells (scale bar 10 µm). Nuclei are stained with DAPI (blue). i Quantitative analysis of the fluorescence intensity of Aβ staining in hypothalamic neurons showing a significant increase in 6- and 12-month-old APP/PS1 mice (n = 2–6 mice per group). Data are expressed as mean ± SD. **p < 0.01; ***p < 0.001; ****p < 0.0001 using two-way ANOVA and Bonferroni’s multiple comparison post-test. wt wild type