Fig. 3

Suppression of invasion of mouse glioma cells by MET inhibition in vitro. Invasiveness was assessed in Corning biocoat matrigel invasion chambers in the absence or presence of tepotinib as pre (8 h)- and co-treatment (100 nM) and without or with irradiation at 2 or 8 Gy. At 24 h after irradiation, equal number of viable cells were re-suspended in fresh serum-free DMEM supplemented or not with tepotinib. The mean number of invading cells was determined 22 h later (⁺p < 0.05, effect of irradiation; ^#p < 0.05, effect of tepotinib). The scale bar represents 100 µm