Fig. 1

HGF/MET pathway activity in murine glioma cells. A,B. Hgf and Met mRNA expression were assessed by RT-PCR in cell lines, tumor or healthy brain tissue (^* p < 0.05, ^** p < 0.01, ^ѲѲ p < 0.01 tumor versus healthy brain). C,D. HGF protein release and constitutive or HGF-stimulated p-MET levels were assessed by ELISA (HGF) (C) or immunoblot (p-MET) in cell lines in vitro (D). The intensities of protein bands relative to actin were quantitated using ImageJ Gel Analysis. E. SMA-497 or SMA-560 cells were exposed to tepotinib to determine time- and concentration-dependent inhibition of MET phosphorylation. F. SMA-560 cells were exposed to tepotinib at 100 nM or HGF at 50 ng/ml or both for 12 or 24 h and MET phosphorylation was determined by immunoblot