Fig. 5

Microglia morphology is altered by extrinsic Nf1 reduction A: Left, 3-dimensional renderings of example male and female WT and Nf1^flox/wt; hGFAP-Cre microglia in the somatosensory and parts of the motor cortex (layer 2–6). Scale bars denote 20 µm. Right, Sholl analysis of male (top) and female (bottom) WT and Nf1^flox/wt; hGFAP-Cre microglia. The number of intersected processes is plotted against their distance from the soma. There were significant differences in the intersected processes between the male WT and Nf1^flox/wt groups. B-D: Summary of the number of intersections per cell (D), the total process length per cell (E) and the soma volumes (F) for male and female WT and Nf1^flox/wt; hGFAP-Cre microglia. Number of quantified cells (mice): 50 (3) for each group