Fig. 3

Microglia morphology is altered in male Nf1 ± mice A: Representative confocal microscopic images (left) and 3-dimensional rendering (right) of a male WT and Nf1 ± microglial cell in the cortex (layer 2–6). Scale bars denote 20 µm. B: Sholl analysis of male (top) and female (bottom) WT and Nf1 ± microglia. The number of intersected processes was plotted against their distance from the soma. Male Nf1 ± cells have a significantly reduced number of Sholl intersections in a radius of 5–40 μm around the soma compared to male WT microglia. C: Summary of the number of intersections of male and female WT and Nf1 ± microglia. D: Summary of the total process length of male and female WT and Nf1 ± microglia. E: Summary of the soma volumes of male and female WT and Nf1 ± microglia. Number of quantified cells (mice): 50 (3) for each group