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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Asymmetric dysregulation of glutamate dynamics across the synaptic cleft in a mouse model of Alzheimer’s disease

Fig. 2

Slow glutamate clearance at pre- but not postsynaptic microenvironments in the 6-month-old 3xTg hippocampus. AD Postsynaptic iGluSnFR expression in CA1 dendrites. A Average postsynaptic iGluSnFR responses to 5 pulses (B) and 100 pulses C of electrical stimulation (100 Hz) in six-month-old WT (black) and 3xTg (orange) mice. Grouped data shown in D. E Representative images depicting the postsynaptic iGluSnFR response in 6-month-old WT (top row) and 3xTg (bottom row) mice evoked by 100 pulses of electrical stimulation (100 Hz). FI Presynaptic iGluSnFR expression in CA1 dendrites (F). Average iGluSnFR responses to 5 pulses (G) and 100 pulses H of electrical stimulation (100 Hz) in six-month-old WT (black) and 3xTg (orange) mice. Grouped data shown in I. J Representative images depicting the presynaptic iGluSnFR response in 6-month-old WT (top row) and 3xTg (bottom row) mice evoked by 100 pulses of electrical stimulation (100 Hz). Boxed traces in C and H depict average iGluSnFR traces normalized to the iGluSnFR value at the end of the one-second stimulation. Black lines above iGluSnFR traces indicate the timing and duration of electrical stimulation. Scale bars in B, G: 5%ΔF/F, 200 ms. Scale bars in C, H: 20% ΔF/F, 500 ms. 3xTg traces scaled to match the peak of WT traces. Scale bar in E: 10 µm. Error bars indicate s.e.m. ** p < 0.01, *** p < 0.001

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