Fig. 4

Circ_63706 promotes tumorigenesis and growth of SHH MB cell in vivo. A Expression of circ_63706 in DAOY and ONS76 control (sh-NC) and DAOY and ONS76 with circ_63706-knockdown (sh-circ_63706 #1 and #2) cells. Relative expression to sh-NC is indicated on the y-axis. B Cell viability assays performed with DAOY and ONS76 control (sh-NC) and DAOY and ONS76 with circ_63706-knockdown (sh-circ_63706 #1 and #2) cells. Points represent the mean ± SD of three biological replicates. C DAOY and ONS76 control (sh-NC) and DAOY and ONS76 with circ_63706-knockdown (sh-circ_63706 #1 and #2) cells expressing luciferase were implanted into the cerebellums of NOD-SCID mice, and tumor formation was assessed by bioluminescence imaging. Changes in bioluminescent signal were examined weekly after tumor implantation. D Quantification of total photon counts from mice implanted with DAOY and ONS76 control (sh-NC) and DAOY and ONS76 with circ_63706-knockdown (sh-circ_63706 #1 and #2) cells. n = 5. E Ki67 staining of xenograft tumor sections. Nuclei are stained with DAPI. Scale bars, 50 μm. Quantification of Ki67-positive cells are shown in (F). *p < 0.05, Student’s t-test. G Overall survival was determined by Kaplan–Meier analysis, and the log-rank test was applied to assess the differences between groups. *p < 0.05, Mantel–Cox log-rank test