Fig. 6

AQP4 relocalizes to alternate cellular locations in the SI/NB and hippocampus 12 m after jmTBI. A GFAP-AQP4 double-staining in representative images of perivascular structures corresponding to astrocyte endfeet in contact with blood vessels and astrocyte processes in the SI/NB. B AQP4 quantification within perivascular astrocyte endfeet (left graph) and astrocyte processes (right graph) revealed significant AQP4 reductions in the SI/NB. Two-way ANOVA (# indicates global jmTBI vs sham differences) with Sidack post-hoc test (* indicates jmTBI vs sham differences in the ipsilateral or contralateral side). Data expressed as mean + SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ####P < 0.0001 C Representative images of perivascular structures corresponding to astrocyte endfeet in contact with blood vessels and astrocyte processes in the hippocampal DG from GFAP-AQP4 double stained sections. D AQP4 staining was significantly increased in DG perivascular astrocyte endfeet (left graph) and astrocyte processes (right graph) in the ipsilateral and contralateral regions. E GFAP-AQP4 staining in the hippocampal CA1 perivascular structures corresponding to astrocyte endfeet in contact with blood vessels and astrocyte processes. E AQP4 staining was significantly increased in perivascular structures (left graph) in the ipsilateral CA1 of jmTBI mice. There were no differences in AQP4 from contralateral perivascular structures alongside no changes within astrocytes. Two-way ANOVA (# indicates global jmTBI vs sham difference) with Sidack post-hoc test (*indicates jmTBI vs sham differences in DG or CA1 subregions of the hippocampus). Data expressed as mean + SEM. # or *P < 0.05, ## or **P < 0.01, ###P < 0.001, #### or ****P < 0.0001 (passed Shapiro–Wilk normality test)