Fig. 4

NAD salvage pathway machinery are reduced in RGC relevant layers of the retina and in the optic nerve head. A NAMPT labelling remains detectable across the whole retina in glaucoma but is significantly reduced in the GCC (n = 11 control eyes, 7 glaucoma eyes). B NMNAT1 is significantly reduced in both the GCC and INL in glaucoma and remains stable in the outer retina (n = 11 control eyes, 7 glaucoma eyes). C NMNAT2 is significantly reduced in the GCC in central retina and is otherwise unchanged in the INL and outer retina (n = 11 control eyes, 7 glaucoma eyes. D–F in the ONH, NAMPT, NMNAT1, and NMNAT2 labelling is significantly reduced in the first 500 µm but remain comparable to controls in the proceeding optic nerve (NAMPT: n = 11 control eyes, 5 glaucoma eyes; NMNAT1: n = 10 control eyes, 5 glaucoma eyes; NMNAT2: n = 8 control eyes, 5 glaucoma eyes). GCC ganglion cell complex, INL inner nuclear layer, OR outer retina, ONH optic nerve head, Ctrl control, Glau glaucoma. Scale bars = 50 µm in A–C, 500 µm in D–E