Sporadic Creutzfeldt–Jakob disease infected human cerebral organoids retain the original human brain subtype features following transmission to humanized transgenic mice

Table 3 Passage 2 inocula

Inocula for P2	DPI	IHC	Blot	SD50/mg
MV1-BH (ms)	180	+	+	8.9 × 10⁶
MV1-OH (ms)	609^#	+	+	5.0 × 10⁶
MV2-BH (ms)	421	+	+	3.8 × 10⁷
MV2-OH (ms)	671	+	+	2.8 × 10⁷

P2: passage 2; Characteristics of mice used for second passage inocula including: DPI: days post inoculation to euthanasia; IHC: immunohistochemistry for PrP deposition; Blot: results of western blotting for PrP^Res; SD50/mg: seeding dose 50% as determined by RT-QuIC (see methods); ^#Mouse was euthanized for non-TSE related reasons before developing clear clinical signs

ISSN: 2051-5960