Fig. 2

A Representative images of immunofluorescence stainings of the cerebral cortex, thalamus, and the hippocampus (CA3 subfield) of a wildtype, Grn^−/−, and Grn^−/− × Tmem106b^T186S/T186S with an antibody against CD68 (green). B The quantification of the CD68-positive area/section is indicated (n = 5; Age: 6 months). C Representative images of immunofluorescence stainings of the thalamus of a wildtype, Grn^−/−, and Grn^−/− × Tmem106b^T186S/T186S with an antibody against Iba1 (red). Age: 6 month. D Three-dimensional reconstructions from representative microglia cells of a wildtype, Grn^−/−, and Grn^−/− × ^T186S/T186S mouse stained for Iba1 (red) and CD68 (green) from 50 images in the Z-direction of the thalamus (age: 6 months). E Volumetric quantification of the microglia cell volume of individual cells depicted from 3D reconstructed images. F Volumetric quantification of the CD68-positive phagosome compartment in individual Iba1-positive microglia cells depicted from 3D reconstructed images. B, E, F *p < 0.05; **p < 0.01; ****p < 0.001; ns Not significant