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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: PRC2 disruption in cerebellar progenitors produces cerebellar hypoplasia and aberrant myoid differentiation without blocking medulloblastoma growth

Fig. 5

Loss of PCR2 function accelerated progression of SHH medulloblastomas. A Representative H&E-stained sagittal sections of medulloblastomas in M-Smo, M-Smo/EedcKO, and M-Smo/Ezh2cKO mice at postnatal day 12 (P12) and comparison of cross-sectional area of tumors in midline sections of replicate mice of each genotype, usiong two-tailed Student’s t-test. B Representative IF stains showing H3K27me3 in M-Smo, M-Smo/EedcKO, and MSmo/Ezh2cKO tumors, and H3K27me3 western blots of medulloblastomas from 3 replicate mice of each genotype. C Kaplan–Meier curves compare the survival times of M-Smo, M-Smo/EedcKO, and MSmo/Ezh2cKO mice, using the Log-Rank test. D IHC for cC3 in representative sections, with quantitative analysis of replicate samples, compared by two-tailed Student’s t-test. E IHC for NEUN in representative sections, with western blot of replicate samples quantified on the right, compared by two-tailed Student’s t-test. F–J Flow cytometry analysis of dissociated tumors of indicated age and genotype, showing F, H pRB + fractions and G, I cell cycle distribution of pRB + cells, and J EdU MFI, compared by two-tailed Student’s t-test. *, **, and *** denote p < 0.05, p < 0.01 and p < 0.001 respectively, relative to controls

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