Fig. 2
scRNA-seq shows differences in the composition of EedcKO cerebella, including a population of myoid cells not found in normal brain. A UMAP projection of cells from EedcKO and control cerebella, grouped by transcriptomic similarities into color-coded clusters. B Bubble Plot shows the magnitude and frequency of the expression of indicated cell type markers in each cluster. The genes and clusters are ordered according to the hierarchical clustering analysis, with groupings indicated along the top and right margins of the Bubble Plot. C UMAP projection of cells from EedcKO and control cerebella, disaggregated by genotype, with clusters indicated by the same color code as in (A). D Comparison of each normalized cluster population in EedcKO and control cerebella. Dots represent values for individual replicates, bars indicate the means and whiskers indicate the SEM. *** indicates p < 0.0001,** indicates p < 0.001, * indicates p < 0.05; Dirichlet regression was used to compare normalized cluster populations. E Bar graphs showing the mean proportional population of each cluster in EedcKO and control cerebella, with the subsets of proliferating CGNPs and differentiating CGNPs and CGNs also shown separately for clarity. In B and D, CGNP and CGN cluster numbers are presented in red for clarity