Fig. 1
PRC2 function is developmentally regulated in the postnatal cerebellum and is required for normal development. A–C Representative sagittal sections of A control, B EedcKO, and C Ezh2cKO cerebella at the indicated ages. H&E-stained sections show the distribution of cells in the EGL and IGL. IHC shows distribution of EED, EZH2, and H3K27me3, with DAPI counterstain. Red arrowheads highlight Purkinje cells, identified by their typical large nuclei. White arrow indicates a residual EGL population in P21 Eed knockouts. D Western blot for H3K27me3 and H3K27Ac in replicate samples of indicated genotypes, quantified below, using two-tailed Student’s t-tests. E Western blot for H3K4me3 and H2AK119ub in replicate samples of indicated genotypes, quantified below, using two-tailed Student’s t-tests. F IHC for pRB and cC3 in representative sections, with quantitative analysis using two-tailed Student’s t-tests. *, **, and *** denote p < 0.05, p < 0.01 and p < 0.001 respectively, relative to controls