Fig. 4

Transcriptional changes and glutamate secretion in CIC-depleted human neural progenitor cells. A Human neural progenitor cells (hNPCs) stably expressing Cas9 and sgCIC targeting CIC, as determined by immunoblot. B hNPC differentiation at day 5 after withdrawal of EGF and FGF2, scale bar = 40 μm. C Positively or negatively enriched top five GO terms in CIC-depleted hNPCs, as assessed by the normalized enrichment score of GSEA (q < 0.05). D Volcano plot showing DEGs between CIC-proficient and -deficient hNPCs. Red dots indicate significantly regulated DEGs. E Glutamate concentration in CIC-proficient and -deficient hNPCs with or without growth factors. Mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01