Fig. 6

Co-expression of POPDC1 and POPDC2 is required for plasma membrane localization in HEK293 cells. a POPDC1-ECFP, POPDC2-EYFP, or both were transiently expressed in HEK293 cells. The plasma membrane was marked using DiD. Scale bar: 10 μm. b Bar graph of the ratio of plasma membrane to cytoplasm expression of POPDC1 or POPDC2 (POPDC1-ECFP: n = 15, POPDC2-EYFP: n = 15, POPDC1-ECFP + POPDC2-EYP: n = 46; min., N ≥ 2). Bars show median ± 95% CI. The groups were compared using a Mann–Whitney test; ****p < 0.0001. c POPDC1 V183F-ECFP, Q153X-ECFP and S201F-ECFP and POPDC2 W188X-EYFP constructs were co-expressed with the appropriate wild-type POPDC partner in HEK293 cells. The plasma membrane was marked using DiD. Scale bar: 10 μm. d Bar graph of the ratio of plasma membrane to cytoplasm expression of POPDC1 or POPDC2 in the presence of the different POPDC1 and POPDC2 mutant proteins (WT n = 46, V183F n = 47, Q153X n = 17, S201F n = 22, W188X n = 24, min., N ≥ 2). Identical data are shown in b and d for the expression levels after co-transfection of both wild-type constructs. Bars show median ± 95% CI. Groups were compared using Kruskal–Wallis followed by Dunn’s test using the wild-type pair for comparison; ****p < 0.0001