Skip to main content
Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Differential effects of mutations of POPDC proteins on heteromeric interaction and membrane trafficking

Fig. 4

Intermediate reduction of POPDC1 and POPDC2 expression at the sarcolemma of skeletal muscle fibers of a homozygous Popdc2W188X/W188X knockin mouse. a and b Transverse sections of the m. gastrocnemius of a 3-month-old homozygous Popdc2W188X/W188X mouse mutant (W188X) and a wild-type control (WT) were stained for (A) POPDC1 or (B) POPDC2, along with SGCA as a sarcolemma marker. Scale bar: 100 μm. c and d The expression levels of c POPDC1 and d POPDC2 in the sarcolemma, normalized to SGCA, were quantified in individual fibers. The number of sections (sec), images (img) and fibers (fib) analyzed per group are as follows: WT: POPDC1—1 sec, 3 img, 164 fib; POPDC2—1 sec, 3 img, 143 fib. W188X: POPDC1—1 sec, 4 img, 95 fib; POPDC2—1 sec, 4 img, 93 fib. The median POPDC/SGCA-level in each control biopsy was set to 1. Dashed lines indicate the normalized median and interquartile range. The control and homozygous mutant pairs were compared using a Mann–Whitney test; **** p < 0.0001

Back to article page