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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Differential effects of mutations of POPDC proteins on heteromeric interaction and membrane trafficking

Fig. 2

Membrane localization of POPDC isoforms in muscle fibers expressing the POPDC1 p.V183F variant. a and b Transverse sections of skeletal muscle biopsies from PT1 and PT2 harboring the POPDC1 p.V183F variant and respective matched controls (CT1 and CT2) were stained for a POPDC1 or b POPDC2 along with SGCA serving as a sarcolemma marker. Scale bar: 100 μm. c and d The expression levels of c POPDC1 and d POPDC2 at the sarcolemma were normalized to SGCA and quantified in individual fibers. The number of sections (sec), images (img) and fibers (fib) analyzed per group are as follows: CT1: POPDC1—2 sec, 5 img, 161 fib; POPDC2—2 sec, 5 img, 138 fib. PT1: POPDC1—1 sec, 4 img, 167 fib; POPDC2—1 sec, 4 img, 167 fibers. CT2: POPDC1—1 sec, 13 img, 681 fib; POPDC2—2 sec, 8 img, 339 fib. PT2: POPDC1—3 sec, 20 img, 835 fib; POPDC2—3 sec, 14 img, 453 fib. The median POPDC/SGCA-level in each control biopsy was set to 1. Dashed lines indicate the normalized median and interquartile range. Data were analyzed using Mann–Whitney test; ****p < 0.0001

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