Fig. 4

Ccnd1 ISH staining is increased in the granular layer of 9-month-old HD mice. A In situ hybridization for Ccnd1 mRNA (red) shows increased signal in the anterior cerebellar lobules III-V in 9-month-old HD mice (bottom) compared to littermate controls (top). B Quantification of mean fluorescence intensity in the granule layer of lobule IV/V (white square). Analysis was performed on two measures obtained from five biological replicated per group. At 9 months, HD samples (n = 10, Shapiro-Wilks: W = 0.87, p = 0.11) show a significant 1.7-fold increase of Ccnd1 staining compared to controls (n = 10, Shapiro-Wilks test: W = 0.83, p = 0.03), Wilcoxon rank sum test, p = 0.002, n = 10, matched control and HD samples are indicated by dot color and connecting line