Fig. 8

A Representative images of immunohistochemistry of AQP4 antibody acquired with Axioscan microscope in 2-months-old mice. Scale bar 1000 μm. B Histogram of AQP4 density in 2-months-old mice analyzed through the percentage of pixels, after the same threshold is set for all the region of interests. C Representative images of immunohistochemistry in 2-months-old mice of AQP4 and CD31, blood vessel marker, acquired with confocal microscope. D Representative images of immunohistochemistry of AQP4 antibody acquired with Axioscan microscope in 6-months-old mice. Scale bar 1000 μm. E, F Histograms of AQP4 and GFAP densities respectively in 6-months-old mice analyzed through the percentage of pixels, after the same threshold is set for all the region of interests. NF not fragmented mice, F fragmented mice, LS lateral septum, RSC retrosplenial cortex, MSC motor-sensory cortex, DG dentate gyrus, TH thalamus, HY hypothalamus, BLA basolateral amygdala. The data are mean standard error of the mean (SEM). Each data point represents an individual animal. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.0001 versus control by ANOVA followed by Bonferroni post-hoc test, n = 4 per condition