Fig. 7

A Representative images of immunohistochemistry of iba-1 and 6e10 antibodies acquired with confocal microscope in all the regions analyzed. The process of analysis includes the skeletonization of microglia for the evaluation of cell complexity. A representation of skeletonized cells is shown on the right of the iba-1 images for each brain region. B–H Analysis of the structural complexity of microglia cells by using AnalyzeSkeleton (2D/3D) ImageJ plugin. NF not fragmented mice, F fragmented mice. The data are mean standard error of the mean (SEM). **p < 0.01; ***p < 0.005; ****p < 0.0001 versus control by ANOVA followed by Bonferroni post-hoc test, n = 3 per condition