Fig. 5

A Representative images of immunohistochemistry acquired with Axioscan microscope. Scale bar 1000 μm. Aβ plaques are labeled in green and the nuclei with DAPI in blue. B Histogram of Aβ plaques analyzed through the percentage of pixels, after the same threshold is set for all the region of interests. C Histogram of Aβ plaque number. D Representative images of immunohistochemistry of AT8. NF not fragmented mice, F fragmented mice, LS lateral septum, RSC retrosplenial cortex, MSC motor-sensory cortex, DG dentate gyrus, TH thalamus, HY hypothalamus, BLA basolateral amygdala. The data are mean standard error of the mean (SEM). Each data point represents an individual animal. *p < 0.05; ****p < 0.0001 versus control by one-way ANOVA followed by Bonferroni post-hoc test, n = 4 per condition