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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Hydrogen peroxide induced by nerve injury promotes axon regeneration via connective tissue growth factor

Fig. 7

Ctgf neutralization delays neurotransmission recovery upon sciatic nerve injury. A CMAP amplitude recordings in control gastrocnemius muscles, as well as 18 and 28 days after compression of the sciatic nerve, in the absence or presence of a neutralizing antibody against Ctgf. N = 4, 20 fibers analyzed. *p = 0.038 (18 days), *p = 0.042 (28 days). B Representative CMAP traces of control and crushed muscles 18 and 28 days after injury, w/wo anti-Ctgf intraperitoneal administration, showing polyphasic features in muscles treated with the neutralizing antibody. The red traces show the portions used to calculate the CMAP area, measured from the initial to the terminal deflection back to baseline. C, D Axonal re-growth, monitored by NF (red) and GAP43 (white) immunoreactivity (C), and by Tomato expression (red) in Chat-positive motor axons (D), 12 days (C) and 10 days (D) after nerve transection. SCs are in green (C). Dotted lines indicate the injury site. Nerve re-growth is reduced and less organized when Ctgf is neutralized. Scale bars: 500 µm (200 µm in the magnification)

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