Fig. 4
H2O2 is rapidly produced upon compression of the sciatic nerve and originates from mitochondria. A, B Imaging of H2O2 production in the sciatic nerve of a live, anaesthetized mouse before (A, upper panel) and after (A, middle panel) sciatic nerve crush, and quantification of PF6-AM signal (B, *p = 0.029). The dotted squares indicate the lesion site. Lower panel: PF6-AM fluorescence in response to 1 mM H2O2 (positive control). Scale bar: 500 µm. C, D H2O2 increase following sciatic nerve crush is unaffected by the NOX inhibitor VAS2870. Scale bar: 500 µm. Quantification of PF6-AM signal is shown in D. N = 3, *p = 0.045. E, F H2O2 increase upon sciatic nerve crush is abolished by MitoTEMPO incubation. Scale bar: 500 µm. Quantification of PF6-AM signal in shown in F. N = 3, p = 0.123, ns = not significant