Fig. 3

Ctgf neutralization delays functional and morphological recovery of the NMJ. A Evoked junctional potentials (EJPs) recorded at soleus NMJs treated with α-LTx for 96 h, in the absence or presence of a neutralizing antibody against Ctgf. N = 5, 15 fibers analyzed. **p = 0.00. B Muscles in A were processed for indirect immunofluorescence using fluorescent α-BTx to stain post-synaptic AChRs (red), and combined anti-syntaxin 1A/1B and anti-VAMP1 antibodies to identify the pre-synaptic compartment (green). Asterisks identify degenerated NMJs. Scale bars: 50 µm (10 µm in magnification). C Quantification of regenerated NMJs. N = 5, one hundred NMJs analyzed/muscle. *p = 0.048