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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Hydrogen peroxide induced by nerve injury promotes axon regeneration via connective tissue growth factor

Fig. 2

ECM components, including Ctgf, are enriched at the regenerating NMJ. A Schematic overview of the integration approach used to identify genes critical to regeneration. The H2O2 data set (left) was obtained by polysome sequencing of SCs exposed to H2O2 for 20 and 40 min [10] and the α-LTx time-course (right) as outlined in Fig. 1. B, C Venn diagrams showing the total number of DEGs up- (B) or down- (C) regulated in the toxin and in the H2O2 data sets, the DEGs in common (169, 85 up vs 84 down), and the relative gene ontology and REACTOME pathway enrichment analysis. The number of genes associated with each category is displayed on the right of the bars (GO_BP: biological process, GO_CC: cellular component, GO_MF: molecular function). D, E RNA seq (D) and digital PCR (E) quantification of Ctgf mRNA levels during the time-course of α-LTx treatment, normalized to control. The reported significant differences were determined by differential expression analysis. N = 5, *p = 0.010, **p = 0.002, ***p < 0.001

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