Fig. 1

NMJ transcriptional profiling during MAT degeneration and regeneration. A Project workflow. CD1 mice were locally injected with α-LTx in the hind limb, and soleus muscles collected 4, 16, 72 and 168 h post-treatment. Contralateral muscles received saline only. Before muscle collection, fluorescent α-BTx was locally injected to label the post-synaptic compartment. Muscles were frozen, cryosliced, and the NMJs collected by laser microdissection and pooled. RNA was extracted, retro-transcribed, amplified and sequenced. B Multi-dimensional scaling analysis (MDS) based on the average expression values of all the expressed genes for each time point. Arrows highlight the circular trajectory, which is consistent with MAT degeneration and regeneration. C Differentially expressed genes (DEGs) down- (left) and up-regulated (right) with respect to controls at the four time points analyzed (4, 16, 72 and 168 h)