Fig. 3

Comparative analysis of protein changes. A Venn diagram depicting detected proteins that possessed a significantly greater expression in WT (red) and cNEB KO (blue)respectively, as well as those unchanged between experimental groups. B Volcano plot displaying Log2 fold change against Log10 p-value. Dark blue dots indicate FDR (q value) < 0.05 whilst light blue dots indicate p < 0.05 and black dots indicate p > 0.05. The top 10 most significant proteins for each experimental group have been annotated in blue (cNEB KO) or red (WT) using protein names except were due to size constraints gene names were used (MRP-L27, MRCK alpha, FOXRED2, PARP1, DLST, SR-Beta and VEGFR2 (39S ribosomal protein L27-mitochondrial, Serine/threonine-protein kinase MRCK alpha, FAD-dependent oxidoreductase domain-containing protein 2, Poly[ADP-ribose] polymerase 1,2-Oxoglutarate Dehydrogenase Complex Component E2, Signal recognition particle receptor subunit beta, Vascular endothelial growth factor receptor 2, respectively). Myosin binding protein C and H were also annotated and their abundances are highlighted in the volcano plots in C, D. E A heat map was created to illustrate the proteins with the greatest fold change, all proteins included possessed a Log2 FC > 1.5. For readability gene names rather than protein names were included in the heap map the conversion between gene name and protein names can be found in Additional file 1: Table S2. ***Indicates p < 0.001