Fig. 6

Microgliosis in the retina of P310S mice. A, B Quantification of cone PRs and RGCs in WT and HE-P310S mice, at 6 and 9 months of age, following immune labeling with the cone PR arrest in antibody and the RPBMS antibody, respectively. C–H Distribution and quantification of retinal microglia immuno labeled with the Iba-1 antibody (red) in whole-mount retinas from WT (C, F) and HE-P310S (D, G) mice aged six (C, D) and nine (F, G) months. E, H Quantification of retinal microglia in WT and HE-P310S mice aged six (E: *p = 0.05; unpaired Mann–Whitney t test) and nine (H: *p = 0.02; unpaired Mann–Whitney t test) months. I–N Microglial cell localization (red) with respect to the distribution of p-tau protein, as determined with the AT8 antibody (green) on a retinal flat-mount (I, K, L) and a retinal section (J, M, N). White arrowheads indicate microglia aligned with AT8-immunopositive RGC axons (I, L). Scale bars: 80 µm in A–G, 50 µm in I–N