Fig. 1

Tau-protein phosphorylation profile in the retina of 9-month-old P310S mice. A–D, I Immunolabeling performed with: the AT8 antibody detecting Ser202-phosphorylated tau protein; E–G, J the PHF1 antibody targeting the double (Ser396/Ser404)-phosphorylated tau protein; K the monoclonal MC1 antibody directed against the conformational epitopes formed by amino acids 7–9 and 313–322 and L the AT100 antibody, recognizing the double-phosphorylated residues Ser214 and Thr212. A, B, E, F Tau-protein labeling in whole-retina flat-mounts; C, G a transverse section of the optic nerve; D sagittal section of the visual cortex and H–L transverse sections of the retina. B, F Represent magnifications of the insets in A and E, respectively. All retinal flat-mounts show the labeling of the retina nerve fiber layer (RNFL) (A, B, E, F, in green) with the labeled fibers in C, G of the optic nerve (ON). (D, white box) The representative brain section shows neuronal labeling in the visual cortex. I–L Retinal sections illustrate the rather even immunolabeling of RGCs and the more variable labeling of the outer retina up to the PR level (I–K). The scale bars represent 100 µm