Fig. 5
From: Wolfram syndrome 1b mutation suppresses Mauthner-cell axon regeneration via ER stress signal pathway
Complementation of wfs1b at single-cell electroporation promotes M-cell axon regeneration. a Construction of the wfs1b expression system. Plasmids express only mCherry served as the control vector. b Timeline of time points of electroporation, axotomy, and regeneration imaging. c Schematic of M-cell soma electroporation. d Confocal imaging of zebrafish larvae 12 h after electroporation (far left) and magnified images of the brain stem in zebrafish larvae, denoting the position of M-cell soma under different fluorescence in the white box. mCherry represents the axons that were labeled by mCherry. Scale bar, 50 μm. e, f wfs1b gene retro-complementation rescued the length of Mauthner cell axon regeneration (control: 233.1 ± 22.65 μm, n = 10; wfs1b overexpression: 341.4 ± 37.92 μm, n = 9). White asterisk: ablation point. scale bar, 50 μm. P = 0.0225. Assessed by unpaired t test