Table 3 Methods use for immunofluorescence marker quantification and analysis
Marker | Areas assessed | ROIs per area | Statistics used for analysis |
|---|---|---|---|
6E10 (Aβ) | Somatosensory cortex Dorsal hippocampus | 1 | Mann-Whitney test |
Aβ40-SMA/SMA (Vascular Aβ) | Somatosensory cortex Hippocampus Thalamus | 3–4 | Linear mixed-effect model (LME) |
GFAP (Reactive astrocytes) | Somatosensory Cortex Hippocampus | 3 | Linear mixed-effect model (LME) |
Iba-1 (Activated microglia) | Somatosensory Cortex Hippocampus | 3 | Linear mixed-effect model (LME) |
NeuN (Neuronal cell loss) | Hippocampal distal CA1 | 3 | Linear mixed-effect model (LME) |
c-Fos (Cell activity) | Entorhinal cortex Retrosplenial cortex Hippocampal CA1 | 4 | Linear mixed-effect model (LME) |
WFA (Perineuronal nets) | Entorhinal cortex Hippocampal CA3/CA2 Thalamic reticular nucleus | 1 | Mann-Whitney test |
PV (Parvalbumin positive cells) | Entorhinal cortex Hippocampal CA3/CA2 Thalamic reticular nucleus | 1 | Mann-Whitney test |