Fig. 13
Increased perineuronal nets signal coupled with an increase of PV-positive interneurons in the AD-like aged degu brain. Confocal microscopic views of degu coronal brain sections are aligned as indicated and show focused views of perineuronal nets (PNN) (green), parvalbumin (PV) interneurons (red), and neuronal nuclei (NeuN, blue) in entorhinal cortex (EC, a and d), hippocampal CA2 and CA3 (Hippo, b and e), and thalamic reticular nucleus (TRN, c and f). g–l Quantification shows significantly increased counts of PNN(+)-neurons per mm2 (g) and PV(+)-neurons per mm2 (h) in EC (p = 0.0022 and p 0.0260, respectively) in AD-like degu brains compared to Non-AD controls, along with similar trends for hippocampal CA3/CA2 (p = 0.0931 for PNN; p = 0.3636 for PV) and TRN (p = 0.3939 for PNN; p = 0.5887 for PV). Error bars represent SEM; *p< 0.05, **p < 0.01, n = 6 degus in AD-like and Non-AD groups, n = 3 for male AD-like and female AD-like groups