Fig. 7

Egr1 protein expression in the hippocampus of αSyn-KO mice. Immunofluorescence labeling and confocal microscopy image analysis of hippocampal slices from male and female αSyn-KO mice were performed for Egr1 density. A Representative 20× confocal images for Egr1 (green) and corresponding segmentations using Imaris spot analysis (CA1, magenta; CA2/3, grey; DG; brown). Hippocampal regions are indicated by dashed white lines while Egr1-positive neurons are indicated by spots. C, D Cell density analysis of Egr1-expressing neurons in CA1 (B), CA2/3 (C) and dentate gyrus (DG; D). Histogram bars represent mean ± SEM, ★P < 0.05, n = 6 mice/sex, 2 slices/mouse