Fig. 5

Loss of PCs in CTCF-cKO mice. a typical picture of the whole brains of control and CTCF-cKO mice at P180. The size of the cerebellum in CTCF-cKO mice was smaller than that in control mice. b Sagittal sections of the cerebellum stained with anti-calbindin (green) and counterstained with DAPI (blue) at P180. The left panel shows that CTCF-cKO mice had smaller cerebellums than control mice. The right panel shows none of the calbindin-positive PCs in CTCF-cKO mice. c Temporal analysis of PC loss. The cerebellum was stained with anti-calbindin (green) and anti-active caspase-3 (red) and counterstained with DAPI (blue). PCs were dramatically lost from P90 to P120 in CTCF-cKO mice. We observed no staining differences for active caspase-3 between control and CTCF-cKO mice. d Quantification of the number of PCs. n > 4 at each stage for each genotype. **p < 0.01, ***p < 0.005. Error bars represent the SEM. Scale bars: 5 mm (a), 500 μm (b, left panel), 100 μm (b, right panel and c)