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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: CTCF loss induces giant lamellar bodies in Purkinje cell dendrites

Fig. 1

Grid2-Cre-mediated CTCF-cKO mice show progressive motor coordination deficits. a Confirmation of CTCF deletion by immunohistochemical staining of the cerebellum at P21. Positive anti-CTCF signals (magenta) were not detected in the PCs of CTCF-cKO mice (yellow arrowheads) or some molecular layer neurons (white arrowheads). Scale bars: 100 μm. b The body size of CTCF-cKO mice was smaller than that of control littermates. Mice at P30 are shown. c Body weight over time from P7 to P35 was measured for each genotype. n = 11 Ctcf;Grid2-Cre (+/fl; +/+), n = 8 Ctcf;Grid2-Cre (fl/fl; +/+), n = 9 Ctcf;Grid2-Cre (+/fl; +/Cre), n = 11 Ctcf;Grid2-Cre (fl/fl; +/Cre). Significant differences were found between Ctcf;Grid2-Cre (fl/fl; +/Cre) and other genotypes at P28 and P35. d Footprint analysis. Representative footprint patterns of control and CTCF-cKO mice at P60. Black, forepaws. Orange, hindpaws. Print separation and stride length and width are defined as indicated (right panel). Scale bars: 1 cm. e Print separation (left), front footprint ratio (width/length of forepaw, centre), and hind footprint ratio (width/length of hidpaw, right). n = 10 (control, cKO). f Footprint analysis. Representative footprint patterns of a control and CTCF-cKO mouse at P180. Orange, forepaws. Black, hindpaws. Scale bars: 1 cm. g–j Rotarod test. A total of six trials (T1–T6) were conducted for each mouse at P56–62 (g, h) and P175–182 (i, j). (g, i) In one set of trials, rod rotation occurred at a constant rate of 5 rpm over a 60-s period. (h, j) In a second set, rod rotation accelerated from 0 to 3 rpm over the first 60 s, and the mice were measured for a maximum of 120 s. g n = 11 (control), n = 19 (cKO); h n = 9 (control), n = 12 (cKO); i, j n = 6 (control), n = 7 (cKO). n.s., not statistically significant; *p < 0.05, **p < 0.01, ***p < 0.005. Error bars represent the SEM

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